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KMID : 1234420100380020183
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Korean Journal of Microbiololgy and Biotechnology
2010 Volume.38 No. 2 p.183 ~ p.189
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Screening of α-Amylase and α-Glucosidase Inhibitor from Nepalese Plant Extracts
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Kim Mi-Seon
An Seon-Mi
Jung In-Chang
Kwon Gi-Seok
Sohn Ho-Yong
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Abstract
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In the course of screening for anti-acidosis and anti-diabetes agent from natural products, the inhibitory activities of Nepales plant extracts against microbial α-amylase and α-glucosidase were evaluated. Among the 100 different kinds of ethanol extracts, Cedrus deodara (Roxb.) G. Don and Myrica nagi Thunb showed strong inhibition activities against α-amylase. The IC50 values of C. deodara (Roxb.) G. Don, M. nagi Thunb and acarose, a commercial available anti-diabetes agent, were 44.5, 47.5 and 50.5 μg/mL, respectively. Considering the crude extract of C. deodara (Roxb.) G. Don, and M. nagi Thunb, these extracts have strong potentials as anti-acidosis or anti-diabates agent. In a while, Cleistocalyx operculatus (Roxb.) extract showed a good inhibition activity to α-amylase and α-glucosidase, even it was recently reported. The selected three extracts did not show any hemolysis activity against human red blood cell up to 1 mg/mL, and the inhibition activities were maintained by heat or acid treatment. Moreover, treatment of HCl (0.01N) for 1 h into C. operculatus (Roxb.) and M. nagi Thunb increased the inhibition activity from 50% to 70%. Our results suggest that C. deodara (Roxb.) G. Don, M. nagi Thunb, and C. operculatus (Roxb.) are potential as anti-acidosis and anti-diabetes agents.
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KEYWORD
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Cedrus deodara, Cleistocalyx operculatus, Myrica nagi, nepalese plant extract, α-amylase ingibitor, α-glucosidase inhibitor
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